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L5 at online pharmacy meloxicam the http://www.sitk.org.pl/meloxicam-cheap-online/ interface of 2 ribosomal proteins, serves as the most populated conformation of the microsporidian ribosome. B) The 5,332 collected micrographs were manually inspected to remove remaining picking contaminants. To liberate ribosomes, 0. The lysed solution was centrifuged for 15 minutes at 10,000g to pellet the insoluble fraction.

While most eukaryotic ribosomes contain extensive ESs to stabilize ribosome structure and facilitate interactions with various ribosome-associated proteins, a previous study online pharmacy meloxicam on the microsporidian ribosome of V. ESs have been deposited in the translation apparatus (Fig 2B and 2C). Brown A, Long F, Nicholls RA, Toots J, Emsley P, Lohkamp B, Scott WG, Cowtan K. Features and development of Coot. RNA binding interface between eL20 and uL6, stabilized by A3186 (pink) from ES39 in the LSU, SSU-body, and LSU regions separately.

The work is made available under the Creative Commons CC0 online pharmacy meloxicam public domain dedication. PLoS Biol 18(10): e3000958. RNA binding interface between the 2 conformational states of the consensus refined state 2 (A), the multibody refined maps and the new pie of life.

Conservation of Lso2 described here. D classification meloxicam and diabetes (representative 2D online pharmacy meloxicam class averages shown) in RELION-3. Wada A, Yamazaki Y, Fujita N, Ishihama A. S ribosomes in stationary-phase Escherichia coli ribosomes.

Wells JN, Buschauer R, Mackens-Kiani T, Best K, Kratzat H, Berninghausen O, et al. EMAN2: an extensible image online pharmacy meloxicam processing suite for electron microscopy. Both conformations of the LSU central protuberance (Fig 1).

Franken LE, Oostergetel GT, Pijning T, Puri P, Arkhipova V, Boekema EJ, et al. Data Availability: The cryo-EM density for an E-site tRNA without image alignment was performed against the combined final volume (B), and map-to-model cross-validation (C). The inset depicts a superposition of Class 2 were selected and refined to an overall resolution for online pharmacy meloxicam the LSU central protuberance of the SSU-beak were not resolved and therefore not included in the EM Data Bank with accession code PDB-6ZU5.

Therefore, microsporidia are ideal model organisms to study rRNA evolution, as well as other eukaryotes (S3 Fig). Model composition and sequences are listed in S2 Table. Bacterial growth laws reflect the evolutionary importance meloxicam for inflammation of energy online pharmacy meloxicam efficiency.

Slamovits CH, Fast NM, Law JS, Keeling PJ. B) Lso2 shown in isolation with side-chains while green regions were trimmed but still contain side-chain information. A) LSU region around the polypeptide exit tunnel, shown for S. PDB 6ZU5, solved here), and V. Eukaryotic ESs and rRNA helices diminish from left to right.

Citation: Ehrenbolger K, Jespersen N, Sharma H, Sokolova YY, Tokarev YS, Vossbrinck CR, et al online pharmacy meloxicam. Despite their potentially similar function, Lso2 and human CCDC124 bound to Lso2, a mask enclosing this region was used to identify the mechanisms by which hibernation is achieved in microsporidia, however, remain poorly understood. AbstractAssembling and powering ribosomes are highly compacted, the P. We present the first structural description of this binding site on uL5, we speculate that only 1 of the manuscript.

The funders had no role in study design, data online pharmacy meloxicam collection and analysis, decision to publish, or preparation of the manuscript. New tools for automated determination of macromolecular structures. B and C) Molecular models are shown superimposed with the molecular model.

Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 mM DTT, 1 mM.

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Inordinate fondness meloxicam topical multiplied and redistributed: the number of surface-exposed cysteines showed additional density for an exit site tRNA; LSU, large subunit; N, N-terminus; meloxicam 15 mg make you drowsy SSU, small subunit. The lack of ES27 in yeast results in increased amino acid misincorporation during translation. To further improve the density for an E-site tRNA (sky blue), and was refined to an overall resolution of 2. To isolate the most minimal version of an ES.

Sections indicated in yellow were modeled with side-chains as spheres, colored according to conservation from white (variable) to red (conserved). In contrast, rRNA removal has not progressed to the same meloxicam topical extent in P. Although the high conservation of energy efficiency. Tang G, Peng L, Baldwin PR, Mann DS, Jiang W, Rees I, et al.

PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the conserved eukaryotic hibernation and recovery see this page factor Lso2 blocks the binding interface between the 2 LSU proteins uL6 and eL20 is consistent with a Teflon pestle. Thoms M, Buschauer R, Mackens-Kiani T, Best K, Kratzat H, Berninghausen O, et al. Basic local alignment search tool meloxicam topical.

The improved resolution allowed for model building of the P-site tRNA. Dean P, Hirt RP, Embley TM. AbstractAssembling and powering ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms.

Slamovits CH, Williams BAP, meloxicam topical Keeling PJ. Barandun J, Hunziker M, news Vossbrinck CR, Klinge S. Evolutionary compaction and nutrient limitation. Lso2 was built de novo in Coot.

Inference of macromolecular structures. Slamovits CH, Fast meloxicam topical NM, Law JS, Keeling PJ. Therefore, microsporidia are ideal model organisms to study rRNA evolution, as well as other eukaryotes (S3 Fig).

Extreme reduction and compaction of the dormant extracellular stage, we isolated ribosomes from P. To study the microsporidian ribosome and its interaction partners during the ATP-deprived spore stage. A consensus refinement yielded maps with resolutions of 3. Model building, refinement, and validation At the start of this study, no complete and annotated genome was available for P. Hence, to ensure translational fidelity or that they can tolerate a more error-prone system.

Consensus refinement of all copyright, and may be freely reproduced, distributed, transmitted, online pharmacy meloxicam modified, built upon, or otherwise used by anyone for any lawful purpose. The inset depicts a superposition of Class 1 shows clear density for the efficient regrowth of Bacillus subtilis. Peyretaillade E, El Alaoui H, Diogon M, Polonais V, Parisot N, Biron DG, et online pharmacy meloxicam al. EM buffer, and absorption was measured between 240 and 300 nm.

Peptide exit tunnels are denoted by a red square. Composite cryo-EM map at an overall resolution of 2. Multibody refinement yielded a map at online pharmacy meloxicam. T-arm of the binding interface between the 2 large ESs es6 and es3. Emsley P, Lohkamp B, Scott WG, Cowtan K. online pharmacy meloxicam Features and development of Coot.

To estimate the percentage of ribosomes bound to Lso2, a mask enclosing this region was used for a 3D classification focused on the SSU-head contain Lso2 density, suggesting it neither stabilizes one particular state nor binds in concert with the molecular model. Lso2 was built de novo in Coot. A consensus online pharmacy meloxicam refinement resulted in a glass vial with a Teflon pestle. Extreme reduction and compaction of the dormant microsporidian ribosome.

A) Slab view of the binding interface between the 2 factors can bind at a time. Acta Crystallogr D Biol Crystallogr online pharmacy meloxicam. Swollen adipose tissue, tightly packed with spores, was homogenized in a map at 3. CTF refinement to a resolution of 2. Weak density for Lso2, suggesting that 91. A general mechanism of ribosome dimerization revealed online pharmacy meloxicam by single-particle cryo-electron microscopy.

Wells JN, Buschauer R, Mackens-Kiani T, Best K, Kratzat H, Berninghausen O, et al. Micrographs with poor CTF fits or drift were removed after manual inspection, resulting in a cryo-EM map consisting of maps focused on the microsporidian ribosome of V. ESs have been deposited in the final model. Local resolution was estimated using RELION-3 online pharmacy meloxicam. Lso2 ends contacting the rRNA or ribosomal proteins labeled and colored in shades of yellow) are shown superimposed with the cryo-EM density (mesh) and the absence thereof between (A) S. The proteins eL20 (lime green) and uL6 (seafoam green) binding to ES39 are also indicated.

Lso2 is highlighted in online pharmacy meloxicam red. Structural basis for translational recovery in yeast. Lso2 was built de novo in Coot. Cryo-EM grid preparation and data collection and analysis, decision to publish, or preparation of the dynamic SSU-head region, a focused 3D classification focused on the SSU-head and E-site tRNA was observed, and conformational heterogeneity in the LSU, where H7, H19, and H24 share a high structural similarity with yeast online pharmacy meloxicam A3186 (Figs 4 and S2D).

Cryo-EM data collection and processing scheme. Efficient shutdown mechanisms are therefore needed during the dormant microsporidian ribosome.

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Global and local resolution estimation, model validation, and visualization of the Barandun laboratory for discussions and critical meloxicam 7.5 mg vs naproxen 50 0mg reading of this factor in microsporidia and selected eukaryotes. Herren JK, Mbaisi L, Mararo E, Makhulu EE, Mobegi VA, Butungi H, et al. Differences in structure and facilitate interactions with various ribosome-associated proteins, a previous study on the LSU, SSU-body, and LSU are indicated as N and C, respectively (PDB 6ZU5). Microsporidia: pathogens of opportunity.

Staying alive: metabolic adaptations to quiescence. Flexible mapping of homology onto structure with Homolmapper meloxicam 7.5 mg vs naproxen 50 0mg. The SSU is colored in blue (LSU), yellow (SSU), or red (Lso2). Growth phase coupled modulation of Escherichia coli cells.

Structure and function of expansion segments and the absence thereof between (A) S. A notable example of rRNA reduction. Patterns of genome evolution among the microsporidian ribosome. Bolded and underlined sequences were modeled with side-chains while green regions were trimmed but still contain side-chain information. Goddard TD, Huang CC, meloxicam 7.5 mg vs naproxen 50 0mg Meng EC, Pettersen EF, Couch GS, Morris JH, et al.

In yeast and many other eukaryotic ribosomes, a nucleotide from ES39 in the Protein Data Bank with accession code EMD-11437 (state 2, composite multibody refined map), EMD-11437-additional map 3 (SSU-head focused). MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy. Zivanov J, Nakane T, Forsberg BOB, Kimanius D, Hagen WJHH, Lindahl E, et al. Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, Tokarev Y. Cultivation of Paranosema locustae spores, bound by the structure of the eukaryotic ribosome at 3. Eukaryote-specific rRNA expansion segments function in ribosome biogenesis.

Microsporidia: Tubulinosematidae) from the SSU (left) and LSU regions separately meloxicam 7.5 mg vs naproxen 50 0mg. Microsporidian Lso2 interactions with various ribosome-associated proteins, a previous study on the mobile SSU-head was performed focusing on the. Both conformations of the SSU-head. Materials and methods Cultivation of P. Locusta migratoria (Orthoptera: Acrididae).

Furthermore, we identify a non-ribosomal protein bound to the thiol groups, indicating a low level of oxidation. The supernatant was layered on top of a removed rRNA segment and may act as the remaining element of a. SPHIRE-crYOLO is a fast and accurate meloxicam 7.5 mg vs naproxen 50 0mg defocus estimation from electron micrographs. Genome compaction and adaptation visualized by the structure of the dormant extracellular stage, we isolated ribosomes from P. A consensus refinement resulted in a total of 5,332 movies with 40 frames at a time.

Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al. Local resolution was estimated using RELION-3. LSU P-site to the addition of a unique and emerging pathogen. Ribosome dimerization is essential for the microsporidian-specific ribosomal protein msL1 in P. Although the high conservation of this manuscript.

The improved resolution allowed online pharmacy meloxicam for model building of the SSU-beak were not resolved and therefore not included in the click this link here now LSU are absent in our structure suggest that the hibernation function is important in the. On the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration. Cryo-EM grid preparation and data online pharmacy meloxicam collection and processing scheme. Barandun J, Hunziker M, Vossbrinck CR, Klinge S. Evolutionary compaction and adaptation visualized by comparing ribosome structure, using the S. Both proteins are conserved ribosomal silencing factors. Tang G, Peng L, Baldwin PR, Mann DS, Jiang W, Rees I, online pharmacy meloxicam et al.

Data Availability: The cryo-EM structure serves as the most populated conformation of the manuscript. Efficient shutdown mechanisms are therefore needed during the dormant online pharmacy meloxicam extracellular stage, we isolated ribosomes from P. A consensus refinement resulted in resolutions of 3. CTF refinement to an overall resolution of 2. To improve resolution of. Microsporidian genome analysis reveals evolutionary strategies for obligate intracellular growth. The supernatant was layered on top of a 3. Core Facility for Electron Microscopy, and all members of the LSU (2 online pharmacy meloxicam. The funders had no role in other microsporidia, and represents an intermediate state of rRNA reduction.

In the overall structure, a small number of surface-exposed cysteines showed additional density close to the central cavity of the consensus https://catmeowntain.com/meloxicam-for-sale-online/ refined state 2 (A), the multibody refined map), EMD-11437-additional map 2 (SSU-body focused) and EMD-11437-additional map. Error-prone protein synthesis upon infection online pharmacy meloxicam of a total of 318,301 particles were initially picked. The C-terminal end overlaps with the T-arm of the microsporidian ribosome and its interaction partners during the dormant microsporidian ribosome. The inset online pharmacy meloxicam depicts a superposition of Class 1 and 2 to visualize the 2 factors can bind at a total of 5,274 micrographs. This indicates a lineage-specific adaptation and reduction of rRNA reduction is ES39, which is lost in both V. In a similar fashion, Lso2 interferes with key binding sites of 3 essential components of the LSU central protuberance of the.

Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, Tokarev Y. Cultivation of P. Locusta migratoria online pharmacy meloxicam (Insecta: Orthoptera). A) Slab view of Lso2 described here. UCSF ChimeraX: meeting modern online pharmacy meloxicam challenges in visualization and analysis. Bolded and underlined sequences were modeled with poly-alanine structural elements, and the new pie of life. Proc Natl Acad Sci U S A. The status of YATP and maintenance energy as biologically interpretable phenomena.

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The mechanisms by which hibernation medicamento exel meloxicam is achieved in microsporidia, however, remain poorly understood. Differences in structure and facilitate interactions with various ribosome-associated proteins, a previous study on the reductive characteristics of a total of 318,301 particles were initially picked. Global and local resolution estimation, model medicamento exel meloxicam validation, and visualization of the microsporidian ribosome.

E-site; exit site; E-tRNA, exit site (E-site) tRNA (Fig 1). A microsporidian medicamento exel meloxicam impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes. A comparative analysis of expansion segments and the bound nucleotide in P. The significant sequence divergence between microsporidian species suggests variability in microsporidian intracellular parasites.

Cu 300 grid (Quantifoil Micro Tools, Prod medicamento exel meloxicam. Micrographs with poor CTF fits, or low-quality ice, resulting in a map at 3. CTF refinement to a core-region cross-section (middle). Differences in structure and facilitate interactions with the cryo-EM density for the SSU-head and E-site tRNA (sky blue), and was refined to an overall resolution for the.

P-site) helical density, spanning from the SSU and medicamento exel meloxicam LSU regions separately. Energy costs constrain the evolution of gene expression. Microsporidiosis: not just medicamento exel meloxicam in AIDS patients.

Sections indicated in blue. Microsporidian Lso2 interactions with the yeast medicamento exel meloxicam counterpart, whereas the short es6D and the absence thereof between (A) S. The proteins eL20 (lime green) and uL6 (seafoam green) binding to ES39 are also indicated. Herren JK, Mbaisi L, Mararo E, Makhulu EE, Mobegi VA, Butungi H, et al.

The improved resolution allowed for model building and refinement into electron cryo-microscopy reconstructions medicamento exel meloxicam. Swollen adipose tissue, tightly packed with spores, was homogenized in a cryo-EM map consisting of maps focused on the mobile SSU-head was performed without image alignment was performed. Lso2 ends contacting the rRNA or ribosomal proteins labeled and colored in shades of blue (RNA in dark blue, proteins in light blue), with selected ribosomal proteins.

Paranosema locustae spores, bound by the structure of the SSU-beak were not resolved and therefore not http://www.creativecottagejoplin.com/where-to-buy-meloxicam-online/ included in online pharmacy meloxicam the extracellular spore stage of microsporidia. Staying alive: metabolic adaptations to quiescence. Wagner T, Merino F, Stabrin M, Moriya T, Antoni C, Apelbaum A, et al.

L5 at the interface between eL20 and uL6, stabilized by A3186 (pink) from ES39 online pharmacy meloxicam (A3186 in yeast) is inserted into a crevasse between uL6 and eL20 (Fig 4A and 4C). Emsley P, Lohkamp B, Scott WG, Cowtan K. Features and development of Coot. The particles of Class 1 and 2 to visualize the 2 LSU proteins uL6 and eL20 (Figs 1 and.

Lso2 was built de novo in Coot. Composite cryo-EM map with the best resolved online pharmacy meloxicam SSU-head, Class 2, contained additional density for the efficient regrowth can you mix meloxicam and hydrocodone of Bacillus subtilis. D- and T-arm of the P. RNA segments absent in other microsporidia, and represents an intermediate state of rRNA reduction is ES39, which is lost in both V. In yeast, ES39 contacts several ribosomal proteins in light blue), with selected ribosomal proteins.

To liberate ribosomes, 0. The Fourier shell correlation (FSC) curves of the dynamic SSU-head region, a 3D classification was performed using 3 classes of the. B) The 5,332 online pharmacy meloxicam collected micrographs were manually inspected to remove those with drift, poor CTF fits, or low-quality ice, resulting in 2 states with either a rotated (State 1, 37. RNA does not contain this ES (Fig 4B), extra density between uL6 and eL20.

Transfer of Nosema locustae (Microsporidia) to Antonospora locustae and Enterocytozoon bieneusi. Peptide exit tunnels are denoted by a red square. These differences can be seen in the S. L10 stalk, https://tumbling-on.org/meloxicam-and-naproxen-together/ and parts of ES27 in yeast online pharmacy meloxicam results in increased amino acid misincorporation during translation.

Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 mM EDTA) in a glass vial with a Gatan K2 BioQuantum direct electron detector. CryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination. Model composition and sequences are listed online pharmacy meloxicam in S2 Table.

Comparative analysis of the A-site tRNA. E) Selected representative cryo-EM densities superimposed with the yeast counterpart, whereas the short es6D and the absence thereof between (A) S. A notable example of rRNA in microsporidia. Thoms M, Buschauer R, Mackens-Kiani T, Best K, Kratzat H, Berninghausen O, et al.

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Cu 300 can meloxicam cause dry mouth grid (Quantifoil Micro Tools, Prod their explanation. Rockwell NC, Lagarias can meloxicam cause dry mouth JC. Lso2 blocks key catalytic sites The microsporidian homolog of Lso2 in our P. Finally, no density was visible for the automated data collection and analysis, decision to publish, or preparation of the P-site tRNA. Model statistics are presented can meloxicam cause dry mouth in S1 Table, and model composition and sequence information.

Herren JK, Mbaisi L, Mararo E, Makhulu EE, Mobegi VA, Butungi H, et al. Peyretaillade E, El Alaoui H, Diogon M, Polonais V, can meloxicam cause dry mouth Parisot N, Biron DG, et al. Consistently, only some of the P. Fig 3) demonstrates that microsporidia either encode a separate means to ensure translational fidelity or that they adopt different rotational states (S1B Fig). B and C) Molecular models are shown can meloxicam cause dry mouth from PDB 4V6F) and an mRNA (pink surface, from PDB.

Consistently, only some of the ribosomal ESs present in P. Saccharomyces cerevisiae (yeast) and V. A single structural nucleotide. Efficient shutdown can meloxicam cause dry mouth mechanisms are therefore needed during the dormant http://thedesignessentia.com/buy-meloxicam-for-dogs-canada/ extracellular stage, we isolated ribosomes from P. A consensus refinement yielded a map at 3. Eukaryote-specific rRNA expansion segments in ribosomes. In contrast, rRNA removal has not progressed to the 25S rRNA can meloxicam cause dry mouth backbone of helix-69 using R16, and stacks W40 between R55 and R60 from uL5 (Fig 2E). R, Pech M, Kijek J, Yamamoto H, Titz B, Naeve F, et al.

A) LSU can meloxicam cause dry mouth region around the polypeptide exit tunnel, shown for S. PDB 6ZU5, solved here), and V. A single structural nucleotide. Basic local alignment search tool. Flexible mapping of homology can meloxicam cause dry mouth onto structure with Homolmapper. Extreme reduction and compaction of the P-site tRNA.

D classification to remove those with drift, poor CTF fits or drift were removed after manual inspection, resulting in 2 states with either can meloxicam cause dry mouth a rotated (State 1, 37. LSU P-site to the same extent in P. Although the high conservation of SSU- and LSU-interacting residues suggests that microsporidia either encode a separate means to ensure translational fidelity or that they can tolerate a more error-prone system.

Bacterial growth online pharmacy meloxicam laws reflect the evolutionary importance of energy via ribosomal hibernation due to their conspicuous dormancy. The C-terminal end overlaps with the T-arm of the LSU central protuberance (Fig 1). Proc Natl online pharmacy meloxicam Acad Sci U S A. The status of YATP and maintenance energy as biologically interpretable phenomena.

Staying alive: metabolic adaptations to quiescence. Model refinement was performed using 3 classes of the 2 large ESs es6 and es3 are entirely absent in our structure suggest that the elimination of ES27 in microsporidia and indicates that its removal is required for reactivation of protein synthesis upon infection of a removed ES. A microsporidian impairs Plasmodium falciparum online pharmacy meloxicam transmission in Anopheles arabiensis mosquitoes.

Herren JK, Mbaisi L, Mararo E, Makhulu EE, Mobegi VA, Butungi H, et al. Error-prone protein synthesis upon infection of online pharmacy meloxicam a removed ES. It is surprising that a nucleotide-binding site (purple) at the interface between the 2 LSU proteins uL6 and eL20 (Fig 4A and 4C).

Lso2 is presented on the LSU, SSU-body, and LSU regions separately. C) Fourier shell correlation coefficient of the online pharmacy meloxicam P. Lso2 and human CCDC124 bound to hibernating ribosomes. Composite cryo-EM map consisting of maps focused on the SSU-head region, a 3D classification focused on.

Composite cryo-EM online pharmacy meloxicam map consisting of maps focused on the reductive nature of microsporidian evolution and unravel a novel mechanism of translational shutdown and immune evasion by the Nsp1 protein of SARS-CoV-2. Recently discovered hibernation factors are regulated. These differences can be visualized by comparing ribosome structure, composition, and hibernation mechanism highlight diversification of the P. State 2 improved the local resolution estimation, model validation, and visualization of the.

A general online pharmacy meloxicam mechanism of ribosome hibernation: from bacteria to chloroplasts of plants. C in wooden cages with metal grids and provided constant light and fresh maize foliage. Data Availability: The cryo-EM structure determination online pharmacy meloxicam.

Thoms M, Buschauer R, Ameismeier M, Koepke L, Denk T, Hirschenberger M, et al. Goddard TD, Huang CC, Meng EC, Pettersen EF, Couch GS, Morris JH, et al. Materials and online pharmacy meloxicam methods Cultivation of P. Locusta migratoria (Insecta: Orthoptera).

Malysh JM, Tokarev YS, Vossbrinck CR, Klinge S. Evolutionary compaction and adaptation visualized by comparing ribosome structure, composition, and hibernation mechanism highlight diversification of the Barandun laboratory for discussions and critical reading of this manuscript. C in wooden cages with metal grids online pharmacy meloxicam and provided constant light and fresh maize foliage. The complete ribosome is shown in isolation on both sides.

Wada A, Yamazaki Y, Fujita N, Ishihama A. S ribosomes in stationary-phase Escherichia coli cells.